Cat. No.
MABL-259
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
9B1
From
Recombinant Antibody
Specificity
This antibody binds morphine and cross reacts with multiple addictive opioids including 6- MAM, morphine, heroin, and hydrocodone, shows weak binding for meperidine, oxycodone and fentanyl without significant binding to currently available OUD treatment agents naloxone, naltrexone, and buprenorphine. Morphine is a strong opiate that is found naturally in opium, a dark brown resin in poppies (Papaver somniferum). It is considered the classic opioid analgesic, most commonly used in pain management, morphine provides major relief to patients afflicted with pain. It is a non-synthetic narcotic with a high potential for abuse.
Alternative Names
Morphium; 6-MAM; 6-monoacetylmorphine; heroin; hydrocodone; opoids; opium; ChEBI: 17303
UniProt
Immunogen
The original antibody was generated by immunizing mouse with 3-carboxymethyl-morphine conjugated to bovine serum albumin.
Application Notes
The original IgG antibody binds morphine with a binding affinity of KD= 33.7nM, and also binds 6-MAM (Kd = 4.8 nM), heroin (Kd = 1.3 nM) and hydrocodone (Kd= 8.2 nM) (PMID: 33356138). The binding ability of this antibody to a variety of drugs was determined using ELISA (PMID: 33356138).
Antibody First Published
Pozharski et al. Anchoring a cationic ligand: the structure of the Fab fragment of the anti-morphine antibody 9B1 and its complex with morphine. J Mol Biol. 2004 Mar 26;337(3):691-7. PMID:15019787
Note on publication
Describes the crystal structure of this antibody in combination with morphine.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
