Cat. No.
MABL-3763
Application
IP, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
bcl-2/124
From
Recombinant Antibody
Specificity
This antibody recognises human BLC-2 which is an antiapoptotic factor in various cell types. Its disregulation was implicated in different conditions, such as cancers and autoimmune disorders.
Alternative Names
Apoptosis regulator Bcl-2; AW986256; B-cell CLL/lymphoma 2; Bcl-2; C430015F12Rik; D630044D05Rik; D830018M01Rik; protein phosphatase 1; regulatory subunit 50; bcl-2- 124; bcl2/124
UniProt
P10415
Immunogen
This antibody was raised against a synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein.
Application Notes
This antibody has been used extensively in attempts aimed at characterising various cancer tissues via immunohistochemical staining. One group used bcl-2-124 antibody to label large B-cell lymphoma samples for bcl2 in order to perform its immunophenotypic and genetic depiction (Chang et al., 2016). Another group took advantage of this antibody while analysing the molecular picture of a case of the cystic synovial sarcoma of the pleura where they observed positive immunohistochemical staining in tumor cells (Tajima et al., 2015). Bcl-2 plays an important role as an inhibitor of apoptosis and its identification is often useful while analysing various factors influencing apoptosis. For instance, bcl-2/124 antibody has been successfully utilised in such experiments identifying bcl-2 via Western blot - Gomez-Bougie (2007) probed the influence of bortezomib on HMCL cells and detected bcl-2 signal on Western blots.
Antibody First Published
Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation. Am J Pathol. 1990 Aug;137(2):225-32. PMID:2201196
Note on publication
This article describes generation and characterisation of the bcl-2/124 antibody.
Size
244 μg Purified antibody.
Concentration
145 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 444.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 164⁰C.
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