Cat. No.
MABL-3774
Application
radiolabelling, therapeutic, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
A5B7
From
Recombinant Antibody
Specificity
This antibody is specific for human CEA.
Alternative Names
CEA; CD66; Q7KZ28; Q13985; Q14081
UniProt
Q13982
Immunogen
This antibody was raised by immunising mice with both carcinoembryonic antigen (CEA) purified from the metastases of colonic tumours, and heat-treated CEA.
Application Notes
In IHC analysis, this CEA-specific antibody stains sections of colon tumour, with high levels of cell surface and cytoplasmic staining of malignant glands, and a more intense reaction in necrotic debris (Harwood et al, 1986). It does not cross-react with PMNs, erythrocytes or liver tissue. In an mouse model transplanted with the human colonic tumour MaWi, the weight of the xenograft has a profound effect on the uptake of a radiolabelled version of antibody (Pedley et al, 1987). In a xenograft tumour mouse model based on the adenocarcinoma cell line LS174T, both this antibody and its FAb have a significant effect on tumour growth when administered as radioimmunotherapeutic agents (Pedley et al, 1993). This antibody has also been shown to function as a radioimmunotherapy agent in a Phase I trial in gastrointestinal adenocarcinomas (Meyer et al, 2009), and has been shown in early clinical trials to target an enzyme capable of cleaving an inactive prodrug to release an active, cytotoxic drug to colorectal cancers (Bagshawe et al, 1991).
Antibody First Published
Harwood et al. Mapping epitope characteristics on carcinoembryonic antigen. Br J Cancer. 1986 Jul;54(1):75-82 PMID:2425837
Note on publication
Describes the original generation of this antibody, and its characterisation in IHC analysis.
Size
255 μg Purified antibody.
Concentration
156 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 455.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 175⁰C.
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