Cat. No.
MABL-3793
Application
WB, Block, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
cet.Hum (Cetuximab - humanized; hC225)
From
Recombinant Antibody
Specificity
This antibody recognizes human EGFR. The epidermal growth factor receptor is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.
Alternative Names
ERBB1; ERBB; ErbB-1, Erb-B1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
UniProt
P00533
Immunogen
The original antibody was generated by grafting CDR loops of cetuximab on framework regions derived from type 3 (VH3 and VL3 kappa) human germline sequences to obtain recombinant VH and VL domains linked together with a flexible linker [(Gly4Ser)3] to form a scFv.
Application Notes
The binding characterization of this humanized scFv version of cetuximab to human EGFR was done using ELISA. This antibody can recognize human EGFR from lysates of A-431 and MCF-7 cells in western blot. The scFv version was able to recognize EGFR on over-expressing cancer cells (A-431) but failed to detect cancer cells with low levels of EGFR (MCF-7 cells) in a flow cytometric experiment. The binding affinity of this scFv version was reported to be 9 fold lower than that of the parental cetuximab, but still it was strong enough for the successful recognition of human EGFR expressing cells. This antibody can be used to block cancer cell surface receptors and inhibit their downstream signaling (PMID:29185855).
Antibody First Published
Banisadr et al. Production of a germline-humanized cetuximab scFv and evaluation of its activity in recognizing EGFR- overexpressing cancer cells. Hum Vaccin Immunother. 2018; 14(4): 856–863. PMID:29185855
Note on publication
Describes the generation of the humanized scFv version of a therapeutic antibody cetuximab.
Size
274 μg Purified antibody.
Concentration
175 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 474.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 194⁰C.
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