Cat. No.
MABL-3809
Application
cytometric bead array, WB, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
C7b
From
Recombinant Antibody
Specificity
C7b specifically recognises HER2 at a different epitope to that recognised by Herceptin. HER2 is a protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone.
Alternative Names
NEU; HER-2; HER-2/neu; ERBB2; CD340; MLN 19; NGL; TKR1; erb-b2 receptor tyrosine kinase 2; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2
UniProt
P04626
Immunogen
A llama was immunized with the HER2-expressing ovarian cancer cell line SKOv3 and a sdAb phage display library was built and used to perform biopannings. The first round of selection was performed on a purified recombinant HER2 ectodomain fused to a human Fc portion (HER2-Fc) followed by a second round on an HER2-positive SKBr3 cell line. After selection, 188 clones were screened and 92 clones were found to be positive by ELISA on HER2 positive cells. Twenty clones were analyzed by sequencing, and all of them corresponded to a unique clone named C7b.
Application Notes
The fine specificity of this antibody was determined by ELISA. The ELISA was preformed using the VHH version of this antibody on HER2 fused to an fc fragment. Furthermore the ability of the antibody to bind to cells expressing HER2 was assessed. This was done by flow cytometry on cells overexpressing HER2 using the VHH version of this antibody (Even-Desrumeaux et al, 2012; pmid:22772166). To assess the reactivity of this antibody, a dot blot was preformed on a ErbB2/HER2 Fc Chimera Protein using the VHH version of the antibody. Further, both a cytotoxicity assay and an internalization assay were preformed using this antibody bound to a model therapeutic nanoparticle (Vorotnikov et al, 2020; pmid:33107540). C7b has also been used to enrich breast cancer cells (SKBr3) from a large excess of human peripheral blood mononuclear cells (Even-Desrumeaux 2012).
Antibody First Published
Even-Desrumeaux et al. Single-domain antibodies: a versatile and rich source of binders for breast cancer diagnostic approaches Mol. BioSyst., 2012, 8, 2385–2394 PMID:22772166
Note on publication
Describes the generation of single-domain antibody fragments against cancer- specific antigens for the use in diagnostic tests for breast cancer, such as ELISA, IHC and microarray multiplex protein measurement by biomarker-based molecule diagnostic and prognostic cancer testing assays.
Size
290 μg Purified antibody.
Concentration
191 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 490.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 210⁰C.
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