Cat. No.
MABL-3827
Application
RIA, SPR, WB, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Cynomologous monkey, Human
Clone No.
K1
From
Recombinant Antibody
Specificity
This antibody can bind the human mesothelium, expressed on many cancer cells like ovarian carcinoma.
Alternative Names
MSLN; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor
UniProt
Q13421
Immunogen
The original antibody was generated by immunization of mice with periodate-treated human ovarian cancinoma (OVCAR-3) cells. These mice had previously been made tolerant to normal human kidney membranes.
Application Notes
This antibody was used for the study and characterization of CAK1 antigen present on ovarian cancers and normal mesothelium. The initial screening of the mesothelin reactive clones to live OVCAR-3 cells was done using immunofluorescence. Secondary screening was done using immunoperoxidase histochemistry on cryostat sections of normal human tissues and human tumors. Quantitative ELISA confirmed that K1 and OC125 antibodies bind to different epitopes on the OVCAR-3 cells (PMID: 1735605). This antibody can detect mesothelin in a western blot assay. Competition RIA was used to detect the presence of CAK1 antigen in the supernatent of protease or phosphatidylinositol-phospholipase C treated cells. An immunotoxin composed of K1 antibody and native Pseudomonas exotoxin was found to be selectively cytotoxic to cells expressing CAK1 antigen (PMID: 1727378). The affinity of K1 antibody to mesothelin was Kd= 8 nM and determined using surface plasmon resonance (BIAcore assay) (PMID: 9178820).
Antibody First Published
Chang et al. Isolation and characterization of a monoclonal antibody, K1, reactive with ovarian cancers and normal mesothelium. Int J Cancer (1992); 50(3):373-81. PMID:1735605
Note on publication
Describes the isolation and characterization of this antibody.
Size
308 μg Purified antibody.
Concentration
209 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 508.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 228⁰C.
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