Cat. No.
MABL-3831
Application
IP, RIA, WB, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
HMFG1 (1.10.F3)
From
Recombinant Antibody
Specificity
This antibody binds the MUC1, a tumor associated antigen expressed on many breast cancer cells. This antibody does not show reactivity towards fibroblasts, lymphoblastoid cells, and a large number of epithelial cell lines of non-breast origin. This antibody recognizes the 'PDTR' epitope of mucin.
Alternative Names
CD227; Muc-1; Mucin-1; Milk mucin; human milk fat globule antigen; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA
UniProt
P15941
Immunogen
The original antibody was generated by immunizing mice with human mammary milk fat globule.
Application Notes
This antibody reacts with most of the breast cancer cells lines and with epithelial cells of human milk. The antibody also reacts with neoplasms of epithelial origin, particularly carcinomas of breast, ovary, cervix, bronchus and gastrointestinal tract. This antibody was used for the study of expression of epithelial antigen in normal and disordered cervical squamous epithelium using immunohistological methods (PMID: 6315048). This antibody can recognize the epithelial membrane antigen in a radioimmunoassay. Immunofluorescent staining of cultures of human breast cells with these antibodies demonstrated that they are heterogeneous in their distribution (PMID: 6086018). HMFG1 and HMFG2 recognize different epitopes of the millk fat globule (PMID: 6086018, 2424847). This antibody was used for the study of tumor associated antigenic epitopes purified from human skimmed milk using immunoaffinity chromatography, immunoprecipitation and immunoblot techniques (PMID: 2443241). The reshaped humanized antibody, HuHMFGl, showed no difference in relative affinity for its antigen compared with the parent mouse HMFG1 (PMID: 7682986).
Antibody First Published
Taylor-Papadimitriou et al. Monoclonal antibodies to epithelium-specific components of the human milk fat globule membrane: production and reaction with cells in culture. Int J Cancer (1981); 28(1):17-21. PMID:7309278
Note on publication
Describes the isolation of this antibody and its used in the study of distribution of the milk mucin antigen on breast cancer cell lines and milk epithelial cells.
Size
312 μg Purified antibody.
Concentration
213 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 512.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 232⁰C.
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