Cat. No.
MABL-3860
Application
Immunoaffinity chromatography, inhibition, IP, RNAseq, WB, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
TK8-14
From
Recombinant Antibody
Specificity
This antibody is specific for human VAP-1, a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. The recognized epitope is present both in dimeric and monomeric forms of VAP-1.
Alternative Names
Vascular adhesion protein 1; VAP1; Copper amine oxidase; HPAO; Membrane primary amine oxidase; Vascular adhesion protein 1; Amine oxidase [copper-containing] 3; Amine oxidase copper-containing 3; Copper amine oxidase; HPAO; Semicarbazide-sensitive amine oxidase 1; Semicarbazide-sensitive; AOC3; Amine oxidase, copper containing 3; amine oxidase; amine oxidase; Vascular adhesion protein 1; SSAO; copper-containing amine:oxy; EC:1.4.3.21
UniProt
Q16853
Immunogen
The original antibody was generated by immunizing specific pathogen-free BALB/c mice with affinity-purified VAP-1 obtained from tonsil stromal lysate.
Application Notes
The original version of this antibody (mouse IgG2a) was generated, and its specificity was characterized by reciprocal immunoprecipitation with another anti-VAP-1 antibody (1B2). In gel analyses, this antibody was shown to react with the dimeric 170-kDa VAP-1 and, under reducing conditions, with the monomeric 90-kDa form of VAP-1. Furthermore, it was used for both immunofluorescence staining and flow cytometry analysis to assess the expression of VAP-1 in rat HEV-like endothelial cell line Ax transfectants (Salmi et al., 1998; PMID: 9605169). The original version of this antibody was biotinylated and characterized via various assays. In an ELISA, it was used to detect serum VAP-1 levels. In a Western blot, it was employed to analyze VAP-1 presence and molecular weight. In immunoaffinity chromatography, it was used to purify VAP-1 from serum samples. In an adhesion assay, it was used to specifically deplete sVAP-1 from serum (Kurkijärvi et al., 1998; PMID: 9686623). This antibody was used in transwell migration assays to study the phenotype of transmigrated monocytes by flow cytometry; it was employed to pretreat primary human hepatic sinusoidal endothelial cells to study its effect on monocyte transmigration.
Antibody First Published
Salmi et al., The role of two distinct endothelial molecules, vascular adhesion protein-1 and peripheral lymph node addressin, in the binding of lymphocyte subsets to human lymph nodes. J Immunol. 1998 Jun 1;160(11):5629-36. PMID:9605169
Note on publication
The original publication explores the distinct roles of two endothelial molecules, vascular adhesion protein-1 (VAP-1) and peripheral lymph node addressin, in mediating the binding of lymphocyte subsets to human lymph nodes. It also describes the generation and characterization of the anti-VAP-1 monoclonal antibody clone TK8-14.
Size
341 μg Purified antibody.
Concentration
242 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 541.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 261⁰C.
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