Key features and details | |
Cat. No. | MABL-524 |
Name | Anti-Biotin mAbs |
Clone No. | AFD- BK-1/39 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | CHIP, IP, FC, IHC |
Species Reactivity | Species-independent |
Basic Information | |
Specificity | This antibody recognises biotin (vitamin B7). It is a water-soluble vitamin which serves as a cofactor of important metabolic enzymes involved in processing of carbohydrates, lipids and amino acids as well as carbon dioxide transport. |
Alternative Name | vitamin B7; vitamin H; coenzyme R |
UniProt | |
Immunogen | This antibody was raised by immunising mice with biotin conjugated to keyhole limpet haemocyanin. |
Application Notes | This monoclonal antibody can be used to detect biotinylated proteins and antibodies, particularly if signal amplification is desired. For instance, PE-conjugated BK-1/39 antibody was used to target biotinylated anti-Ig beta antibody in a flow cytometric analysis (Levit-Zerdoun et al., 2016). This antibody was also successfully utilised in the chromatin immunoprecipitation (ChIP) of biotinylated sense or antisense strands of the saRNA duplex (Wu et al., 2016). BK-1/39 was used in in situ hybridisation as well; it detected biotinylated RNA probe complementary to the Epstein-Barr virus genome (de Lima et al., 2012). |
Antibody First Published | Arnholdt et al. Receptor-mediated processing of epidermal growth factor in the trophoblast of the human placenta. Virchows Arch B Cell Pathol Incl Mol Pathol. 1991;61(2):75-80.PMID:1683723 |
Note on publication | This article describes the earliest found application of BK-1/39 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
