Key features and details | |
Cat. No. | MABL-525 |
Name | Anti-Blood group A antigen mAbs |
Clone No. | AFD- AC1001 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, Hemagglutination assay, Quantitative precipitin assay, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for blood group antigen A. |
Alternative Name | AC-1001 |
UniProt | |
Immunogen | The original antibody was produced by immunizing mice with A1 erythrocytes and with blood group A substance. |
Application Notes | This antibody was used in a quantitative precipitin assay, a hemagglutination assay (HA), inhibition assays (by ELISA). In the HA with human erythrocytes, the antibody reacted with the A1 and A2 subtypes (showing double the sensitivity towards the A1 subtype) (Chen and Kabat, 1985; PMID: 2414276). The structure of the scFv version of this antibody was determined via crystallization (Thomas et al., 2002; PMID: 11679577). |
Antibody First Published | Chen and Kabat. Immunochemical studies on blood groups. The combining site specificities of mouse monoclonal hybridoma anti-A and anti-B J Biol Chem. 1985 Oct 25;260(24):13208- 17 PMID:2414276 |
Note on publication | The original publication explores the specificities of mouse monoclonal hybridoma antibodies targeting blood group A and B antigens. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
