Anti-bicyclo mAbs

Cat. No.

MABL-523

Name

Anti-bicyclo mAbs

Clone No.

AFD- 39-A11

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA   

Species Reactivity

n/a

Basic Information

Specificity

The antibody binds to hapten 4 with an affinity   (Kd) of 10 ± 0.3 nM. The antibody has a Kcat of 0.67 s−1 and a Km of 1200 μM.

Alternative Name

UniProt

Immunogen

Application Notes

The antibody binds specifically to the bicyclo[2.2.2]octen   moiety of hapten 4, and catalyses the pericyclic Diels- Alder reaction. The  Diels-Alder is an organic chemical reaction between a conjugated diene and a   dienophile which results in the formation of a substituted cyclohexene   system. The Monoclonal antibody 39-A11 binds to the diene and dienophile in a   reactive orientation, thereby catalysis the reaction by lowering the   activation energy. The antibody has high affinity for hapten 4, and low   affinity for the reaction end product, allowing it to be released once the   reaction has terminated.

Antibody First Published

Romesberg FE, Spiller B, Schultz PG, Stevens   RC. Immunological origins of binding and catalysis in a Diels-Alderase   antibody. Science. 1998 Mar 20;279(5358):1929-33. PMID:9506942

Note on publication

Describes the generation and   characterization of the three-dimensional structure of the monoclonal   antibody 39-A11, a catalysis in the Diels-Alder reaction.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.