Key features and details | |
Cat. No. | MABL-522 |
Name | Anti-Beta2-Microglobulin mAbs |
Clone No. | AFD- YTH470.5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | activation, Blocking, Positive control, Sensitization, WB, FC, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human β2-Microglobulin. |
Alternative Name | B2M; Beta 2-micro; B2BM; β2M; β 2-micro; β2βM; CDABP0092; HDCMA22P; YTH470; YTH-470 |
UniProt | P61769 |
Immunogen | The original antibody was generated by fusion of the myeloma line Y3/Ag1.2.3 with spleen cells from a DA rat immunized with human cells. |
Application Notes | YTH470.5 was used for IF, Activation (via complement mediated cell lysis), and WB as part of the testing performed in its original article (Bindon et al., 1988; PMID: 2973413). This antibody was also used as a positive control for blocking, as measured by FC, to analyze integrin expression on the surface of primary cultures of human airway smooth muscle cells (Freyer et al., 2001; PMID: 11713098).Furthermore, it was used as a primary monoclonal antibody in the immunocytochemistry analysis — IF, specifically — of human neuronal stem cells (Lee et al., 2005; PMID: 15607744). |
Antibody First Published | Bindon et al. Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies Eur J Immunol. 1988 Oct;18(10):1507-14. doi: 10.1002/eji.1830181006 PMID:2973413 |
Note on publication | Describes the preparation of rat anti human beta2-microglobulin antibody and characterisation of the specificities by binding analysis, immunoprecipitation or Western blotting, furthermore specific functional tests such as complement-mediated lysis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
