Anti-Beta-Tubulin mAbs

Cat. No.

MABL-521

Name

Anti-Beta-Tubulin mAbs

Clone No.

AFD- S11B

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, IF

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human beta-tubulin   (no cross-reactivity with alpha-tubulin). Tubulin is the major constituent of   microtubules. The alpha chain has a non-exchangeable GTP-binding site.   Tubulin is the major constituent of microtubules. The beta-chain has an   exchangeable GTP-binding site.

Alternative Name

TUBB; β-tubulin

UniProt

Q13509

Immunogen

The original antibody was generated   via phage display from the Griffin.1 library by screening against   Microtubule-Binding Proteins (MBPs) derived from HeLa S3 cells.

Application Notes

This antibody's original format   (human scFv) was successfully used in a Western blot, and its anti-myc (9E10) complexed version was successfully used for in vitro immunofluorescence   staining of β-tubulin in HeLa cells (Nizak et al., 2003; PMID: 14617357).

Antibody First Published

Nizak et al., Recombinant antibodies   against subcellular fractions used to track endogenous Golgi protein dynamics   in vivo. Trafficking 2003; 4(11):739-753 PMID:14617357

Note on publication

The original publication   demonstrates the use of recombinant antibodies selected through phage display   against subcellular fractions.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.