Key features and details | |
Cat. No. | MABL-3421 |
Name | Anti-GCN4 mAbs [C11L34](MABL-3422) |
Clone No. | C11L34 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, SPR, WB |
Species Reactivity | S. cerevisiae |
Basic Information | |
Specificity | This antibody binds to the HLENEVARLKK peptide from the leucine-zipper domain of the S. cerevisiae GCN4 transcriptional activator with picomolar affinity. |
Alternative Name | General Control Protein 4 |
UniProt | P03069 |
Immunogen | Biotinylated GCN4-peptide (HLENEVARLKK). |
Application Notes | This antibody binds to the GCN4-leucine zipper peptide, which is part of the dimerisation- and DNA-binding domain of the transcriptional activator GCN4 in S. cerevisiae. |
Antibody First Published | Zahnd et al. Directed in vitro evolution and crystallographic analysis of a peptide-binding single chain antibody fragment (scFv) with low picomolar affinity. Journal of Biological Chemistry 2004; 279(18):18870-18877 PMID:14754898 |
Note on publication | Describes the generation of an scFv with high affinity using directed evolution. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
