Key features and details | |
Cat. No. | MABL-3423 |
Name | Anti-DDDDK-tag mAbs [M2.1](MABL-3424) |
Clone No. | M2.1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, IF |
Species Reactivity | n/a |
Basic Information | |
Specificity | This antibody recognises the DYKDDDDK-tag, a widely used epitope tag. |
Alternative Name | FLAG-tag; Equivalent to FLAG antibodies from Sigma; DYKDDDDK; DDDK; AspTyrLysAspAspAspAspLy |
UniProt | |
Immunogen | Interleukin 2 genetically fused to the peptided DYKDDDDK, corresponding to the DDDDK epitope tag. |
Application Notes | This antibody recognises he widely used DDDDK-tag commonly used as an epitope tag in fusion proteins. The tag can be recognised at any position within a fusion protein, i.e. N-terminally, Met-N-terminally, C-terminally as well as internally. This antibody's binding is not sensitive to calcium. This antibody recognises both native and denatured protein. |
Antibody First Published | Brizzard et al. Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques. 1994 Apr;16(4):730-5. PMID:8024796 |
Note on publication | Describes the characterisation of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
