Anti-TK15 epitope tag mAbs [TK 15](MABL-3426)

Cat. No.

MABL-3425

Name

Anti-TK15 epitope tag mAbs [TK 15](MABL-3426)

Clone No.

TK 15

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, IF

Species Reactivity

Xenopus laevis

Basic Information

Specificity

Recognises recombinant proteins TK15 tag at the carboxy terminus. Amino terminal and internal TK15 tags have not been  tested.

Alternative Name

TK-15 tag

UniProt

Immunogen

Mice were injected with bacterially   expressed full-length Xenopus laevis Orc1p, and spleen cells from three   best-responding mice were used to generate antibody-producing hybridomas.   Antibodies were screened by enzyme-linked immunosorbent assay and immunoprecipitation.

Application Notes

This antibody was first used to isolate Xenopus origin recognition complex peptides (Tugal 1998) but can now be used for the purification and detection of recombinant fusion proteins   incorporating a single eight amino acid TK15 tag.

Antibody First   Published

Tugal et al. The Orc4p   and Orc5p Subunits of the Xenopus and Human Origin Recognition Complex Are   Related to Orc1p and Cdc6p Biol Chem. 273(49):32421-9 (1998) PMID:9829972

Note on publication

Outlines the purification of  Xenopus ORC for characterization of ORC and the study of its interaction with DNA to look for DNA sequence specificity in origin function.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.