Anti-RAP tag mAbs [PMab-2](MABL-3428)

Cat. No.

MABL-3427

Name

Anti-RAP tag mAbs [PMab-2](MABL-3428)

Clone No.

PMab-2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

epitope tag, WB, ELISA, FC, IHC

Species Reactivity

Rat

Basic Information

Specificity

This antibody is   specific for amino acids 38-51 (GDDMVNPGLEDRIEC) of the platelet-   aggregation-stimulating (PLAG) domain of rat podoplanin (PDPN). PDPN is a is a type I transmembrane protein that interacts with the platelet receptor  C-type lectin-like receptor-2 (CLEC-2). PDPN is expressed by a wide variety   of cells, including podocytes, lymphatic endothelial cells and type I   alveolar cells. PDPN is also upregulated on certain cancer cells, where it   mediates tumour-induced platelet aggregation by binding to CLEC-2 on platelets

Alternative Name

Podoplanin; E11 antigen epitope; Glycoprotein 38; Gp38;OTS-8; Ots8; RTI140;T1-alpha; T1A; Type I cell 40 kDa protein

UniProt

Q64294

Immunogen

This antibody was raised   by immunising mice with 14-residue synthetic peptide rpp3851, which   corresponds to amino acids 38-51 (GDDMVNPGLEDRIEC) of the   platelet-aggregation-stimulating (PLAG) domain of rat podoplanin (PDPN).  Spleen cells were then harvested, and fused to P3U1 cells to generate stable   hybridomas.

Application Notes

The specificity of this   antibody for endogenous rat podoplanin (PDPN) has been confirmed in ELISA,   Western blot and flow cytometry analysis (Oki et al, 2015), where PMab-2 was   shown not to react with human and mouse podoplanin. Flow cytometry has also   been used to demonstrate the high binding affinity of this antibody (KD of   PMab-2 = 5.9 × 10−10 M) (Oki et al, 2015). In immunohistochemical analysis,   this antibody is highly sensitive to rat PDPN, detecting type I lung alveolar   cells, colon lymphatic endothelial cells, podocytes and Bowman's capsule   epithelial cells at low concentration (Oki et al, 2015). This antibody   possess high affinity and specificity for the RAP epitope tag (DMVNPGLEDRIE),   and can, therefore, be used to detect proteins in Western blots, flow   cytometry and sandwich enzyme-linked immunosorbent assays (ELISA), as well as   for protein purification. (Fujii et al, 2017).

Antibody First   Published

Oki et al Development of   Sensitive Monoclonal Antibody PMab-2 Against Rat Podoplanin Monoclonal Antibodies in Immunodiagnosis and Immunotherapy.   December 2015, 34(6): 396- 403. PMID:26683179

Note on publication

Describes the original generation and characterisation of this antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.