Key features and details | |
Cat. No. | MABL-3429 |
Name | Anti-Protein C mAbs [HPC-4](MABL-3430) |
Clone No. | HPC-4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, Purification, ELISA |
Species Reactivity | Baboons, Human |
Basic Information | |
Specificity | Specifically binds to a specific twelve peptide sequence (EDQVDPRLIDGK) in the activation region of the Protein C (spanning the thrombin cleavage site). Does not react with activated protein C. Has been shown to be cross-reactive with baboon Protein C but not with bovine or mouse Protein C. |
Alternative Name | Autoprothrombin IIA; C-tag; Protein C tag; Protein C-tag; HPC4; Vitamin K-dependent protein C; Anticoagulant protein C; Blood coagulation factor XIV; PROC |
UniProt | P04070 |
Immunogen | This antibody was raised by immunising mice with human protein C. |
Application Notes | HPC-4 binds human protein C only in the presence of Ca2+; it binds to the reduced carboxymethylated protein C heavy chain, and it blocks protein C activation but does not bind or inactivate protein C or activated protein C bound to its plasma inhibitor. Furthermore, it is an anti-epitope tag antibody, as it recognizes the EDQVDPRLIDGK sequence (Taylor Jr et al., 1987; 3102560). It has been shown to work in ELISAs to determine expression levels (Velander et al., 1992; PMID: 1465430). HPC-4 has been shown to be effective at purifying proteins tagged with its epitope (Rezaie et al., 1992; PMID: 1283093). It has also been used to block the activation of Protein C by the thrombin-thrombomodulin complex (Stearns et al., 1988; PMID: 2447082) and was used for the same purpose in Baboons to lower the threshold of sepsis (Taylor Jr et al., 1987; PMID: 3102560). HPC-4 has been used in many other studies to observe the function of Protein C by knocking out its function in a Ca2+-dependent manner, purification, and others (Tanaka et al., 2006; PMID: 16398653) (Bae et al., 2007; PMID: 17823308). |
Antibody First Published | Taylor et al. Protein C prevents the coagulopathic and lethal effects of Escherichia coli infusion in the baboon. J Clin Invest. 1987 Mar;79(3):918-25. PMID:3102560 |
Note on publication | Describes the generation and characterisation of the HPC-4 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
