Anti-IgE mAbs

Cat. No.

MABL-3360

Name

Anti-IgE  mAbs

Clone No.

AFD-mAbA9

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, ELISA, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for the extracellular membrane-proximal domain (EMPD) of murine membrane-bound IgE.   As this 19-amino acid long EMPD is isotype-specific, this antibody does not   cross- react with soluble mouse IgE.

Alternative Name

mIgE; mouse Immunoglobulin E

UniProt

Immunogen

This antibody was raised by immunising ΔM1M2   BALB/c mice, which cannot express membrane-bound IgE due to the lack of the   IgE-transmembrane and IgE-cytoplasmic domains, with the extracellular   membrane-proximal domain of mouse membrane IgE (EMPD) conjugated

Application Notes

This antibody binds to the extracellular   membrane-proximal domain (EMPD) of mouse membrane-bound IgE with high   specificity and affinity, as confirmed in ELISA analysis and FC analysis   (Feichtner et al, 2008). In mice immunised with recombinant Bet v1a, the major birch pollen allergen, subsequent passive immunization with this   antibody leads to a block of newly synthesized specific IgEs, and reduces   serum Bet v1a-specific IgE titers (Feichtner et al, 2008). In vitro, this   antibody induces receptor-mediated apoptosis in B cells expressing   membrane-bound IgE (Feichtner et al, 2008).

Antibody First   Published

Feichtner et al. Targeting the extracellular membrane-proximal domain of membrane-bound IgE by   passive immunization blocks IgE synthesis in vivo. J Immunol. 2008 Apr   15;180(8):5499-505. PMID:18390733

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.