Anti-IgE mAbs

Cat. No.

MABL-3365

Name

Anti-IgE  mAbs

Clone No.

AFD-D9#3116

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA

Species Reactivity

Dog

Basic Information

Specificity

The antibody recognises canine IgE (likely   C-epsilon 2).

Alternative Name

immunoglobulin E; canine IgE; canine immunoglobulin E; D9

UniProt

Immunogen

This antibody was raised by immunising BALB/c   mice with IgE-rich pooled parasitised dog serum and subsequent creation of hybridomas.

Application Notes

ELISA: Canine IgE-specificity of the D9#3116 antibody was confirmed by ELISA tests with different types of antibodies (DeBoer et al., 1993). WB: This antibody was successfully used in western   blot detection of canine IgE in experiments on the dogs' allergies against   the hen egg (Shimakura et al., 2016). ELISA: fluorometric ELISA tests were   used to assay canine IgE reactivity against different vaccine components in   anti-vaccine allergies (Ohmori et al., 2005).

Antibody First   Published

DeBoer et al. Production   and characterization of mouse monoclonal antibodies directed against canine   IgE and IgG. Vet Immunol Immunopathol. 1993 Aug;37(3-4):183-99. PMID:8236798

Note on publication

Describes the original generation of this antibody, and its use in ELISA analysis.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.