Key features and details | |
Cat. No. | MABL-3369 |
Name | Anti-Asp f 1 mAbs |
Clone No. | AFD-17A5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, ELISA |
Species Reactivity | Aspergillus fumigatus (Neosartorya fumigata) |
Basic Information | |
Specificity | This antibody recognises Asp f 1. |
Alternative Name | A. fumigatus Allergen; Asp f1; Aspf1; ribotoxin Asp f 1; ribonuclease mitogillin; allergen Asp f I; Asp f I; IgE-binding ribotoxin; major allergen Asp f 1; allergen: Asp f 1 |
UniProt | P67875 |
Immunogen | 17A5 antibody was raised using a novel technique: mononuclear cells were taken from the human subjects with allergic bronchopulmonary aspergillosis and then screened for IgE secretion.Subsequently, they were used to generate hybridomas of which some were secreting anti-Asp f 1 IgE. |
Application Notes | 17A5 antibody's target protein (Asp f 1) was confirmed through IP and mass spectrometry analysis (Wurth et al., 2018). WB and ELISA: the antibody was successfull in detecting Asp f 1 antigen in a commercial Aspergillus extract ALKB (Wurth et al., 2018). |
Antibody First Published | Wurth et al. Human IgE mAbs define variability in commercial Aspergillus extract allergen composition JCI Insight. 2018 Oct 18;3(20). pii: 123387. doi: 10.1172/jci.insight.123387. PMID:30333320 |
Note on publication | The paper describes a method of generating Aspergillus-reactive IgE–secreting human hybridomas (among them these secreting this antibody). Subsequently, the antibody is characterized and its specificity is determined. Finally, this antibody is used to determin a potential presence of its antigen in the commercialy-available Aspergillus samples used in diagnosis and treatment of Aspergillus-related allergies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
