Anti-Fel d 1 mAbs

Cat. No.

MABL-3384

Name

Anti-Fel d 1  mAbs

Clone No.

AFD-REGN1909

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallization, functional assay, SPR, Block, ELISA

Species Reactivity

Cat (Felis catus)

Basic Information

Specificity

This antibody binds chain 2 of the Fel d 1, major cat allergen.

Alternative Name

Major cat allergen; CH2; AG4; Major allergen I polypeptide chain 2; Allergen Cat-1; Allergen Fel d I-B; Allergen FdI; Fel dI; Fel d 1-B

UniProt

P30440

Immunogen

The original antibody was generated by immunizing   a Regeneron’s VelocImmune human antibody producing transgenic mouse with   recombinant dimeric Fel d 1.

Application Notes

The original human IgG4   antibody was found to block binding of wild type Fel d 1 binding to IgE with   a maximum blockade of 51% in a blocking ELISA. Surface plasmon resonance was used to identify the affinity of the antibody towards recombinant and wild type   Fel d 1. Crystal structure of the Fab fragment antibody was studied using   X-ray crystallography. This antibody in combination with REGN1908 was found   to be promising in treatment of cat allergies. A single SC dose of   REGN1908–1909 in patients with cat allergy resulted in rapid and clinically   meaningful reduction in total nasal symptoms by blocking the early allergic   response to nasal challenge with cat allergen (PMID: 29650949).

Antibody First   Published

Orengo et al. Treating cat allergy with   monoclonal IgG antibodies that bind allergen and prevent IgE engagement. Nat   Commun. 2018; 9: 1421. PMID:29650949

Note on publication

Describes the generation of this antibody   from a combinatorial IgE library derived from a doner and determines the   clonal similarities between the antibodies generated.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.