Cat. No.
MABL-3866
Application
ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
4D5-8 (trastuzumab)
From
Recombinant Antibody
Specificity
Oncogenic protein Her-2/neu (erbB-2), a member of the family of epidermal growth factor (EGF) receptors, overexpressed in 30% of invasive breast cancers and 70% of ductal carcinomata in situ, in a small percentage of melanomas, and in other malignancies originating from various organs, including ovary, kidney, colon, and bladder.
Alternative Names
CD340; Metastatic lymph node gene 19 protein (MLN 19); Proto- oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; p185HER2; EGFR
UniProt
P04626
Immunogen
A431 cells (human epidermoid carcinoma) (over)expressing EGFR.
Application Notes
This antibody binds to erbB-2/Her-2/neu has therapeutic indications particularly in the treatment of breast cancer (c.f. Herceptin). It can also be used to detect HER-2 by flow cytometry or IHC (Glazyrin 2007).
Antibody First Published
Carter P, Presta L, Gorman CM, Ridgway JB, Henner D, Wong WL, Rowland AM, Kotts C, Carver ME, Shepard HM. Humanization of an anti-p185HER2 antibody for human cancer therapy. Proc Natl Acad Sci U S A. 1992 May 15;89(10):4285-9. PMID:1350088
Note on publication
Describes the humanisation of the antibody (also known as Herceptin), shows it recognised HER2 in a wide variety of immuno-assays.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
![Anti-Beta-galactosidase [13R4]](/public/static/common/images/not_adv.jpg)
