Cat. No.
MABL-3867
Application
FC, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
Cl:A3-1 (recombinant version)
From
Recombinant Antibody
Specificity
This antibody recognises the mouse F4/80 antigen, a 160kD glycoprotein, expressed by murine macrophages.
Alternative Names
mouse macrophage marker; EMR1 receptor; EGF-like module; Cell surface glycoprotein F4/80; EGF-like module receptor 1; Em Gpf4; TM7; Ly-71; f480; f-480; f4 80; f 480; f- 4/80; f4-80
UniProt
Q61549
Immunogen
Thioglycollate stimulated peritoneal macrophages of mouse origin.
Application Notes
The anti-F4/80 antibody, clone Cl:A3-1, has been very widely used in flow cytometry, microscopy and Western blotting since its generation by Austyn and Gordon in 1980. We are the only supplier of recombinant versions of Cl:A3-1 which, whilst having the same variable domains as the original, has been shown to block hybridoma derived anti-F4/80:APC binding to in vitro generated bone marrow- derived (BMDMs) and ex vivo splenic murine macrophages. This was tested using both the recombinant version of the original isotype (rat IgG2b) as well as a murinised version (IgG2a, Ab00106-2.0). Further testing on BMDMs shows that our Fc Silent™ rat IgG2b is a valuable new reagent which shows distinct labelling of F4/80-positive cell populations, whilst the isotype control shows no binding, suggesting that only the antigen binding domains are involved in binding, promoting precision of labelling.
Antibody First Published
Austyn JM, Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur J Immunol. 1981 Oct;11(10):805-15. PMID:7308288
Note on publication
Describes the making of the antibody and its specificity for macrophages (Flow Cytometry) and proposes its potential use as a marker.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
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