anti-c-erbb-2-fwp51-mabl-3765

Cat. No.

MABL-3765

Application

fusion, IP, FC, IF

Isotype

Engineer antibody

Species Reactivity

Human

Clone No.

FWP51

From

Recombinant Antibody

Specificity

FWP51 binds to the extracellular domain of c-erbB-2.

Alternative Names

CD340; Receptor tyrosine-protein kinase erbB-2; Neu; HER2; MLN19; Metastatic lymph node gene 19 protein; p185erbB2

UniProt

P04626

Immunogen

20x10^6 SKBR3 human breast tumour cells were injected into BALB/c mice. The hybridomas generated from the mice spleen cells were then screen for antibodies reactive to c-erbB-2 using immunofluorescence and immunoprecipitation.

Application Notes

Immunofluorescence and immunoprecipitation were used to confirm the specificity of FWP51 for c-erbB-2 (Harwerth et al, 1992). Flow cytometry showed that FWP51 binds the extracellular domain of c-erbB-2 (Harwerth et al, 1992). FWP51 has also been fused to the bacterial toxin, exotoxin A. The resultant fusion protein Fv(FWP51)-ETA selectively inhibited protein synthesis in cells expressing high levels of human c-erbB-2 protein, but not in cells expressing little to no human c-erbB-2 protein.Administration of FWP51 to mice impanted with human erbB-2 transformed NIH3T3 cells led to inhibition of the onset of tumour growth (Harwerth et al, 1993).

Antibody First Published

Harwerth et al, 1992. Monoclonal antibodies against the extracellular domain of the erbB-2 receptor function as partial ligand agonists. J Biol Chem. 1992 Jul 25;267(21):15160-7. PMID:1353079

Note on publication

Describes the original production of the antibody and its characterisation through immunofluorescence and immunoprecipitation.

Size

246 μg Purified antibody.

Concentration

147 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 446.

Storage Recommendation

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 166⁰C.

Anti-c-erbB-2 [FWP51] (MABL-3765)

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