Cat. No.
MABL-3768
Application
IP, WB, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
VK-8-1.9
From
Recombinant Antibody
Specificity
The antibody binds CA125 (mucin 16). CA125 is a glycoprotein from the mucin family and is an important tumor marker or biomarker that may be elevated in the blood of some patients with specific types of cancers, or other conditions that are benign.
Alternative Names
CA-125; CA 125; MUC16; MUC-16; Mucin-16; Ovarian cancer-related tumor marker CA125; Ovarian carcinoma antigen CA125; VK8; VK-8
UniProt
Q8WXI7
Immunogen
The original mouse antibody VK-8 was generated by immunizing BALB/c mice with ovarian cancer cell line OVCAR-3 cells. This antibody is a recombinant version derived from the murine parent antibody VK-8.
Application Notes
This recombinant antibody is derived from the parent mouse IgG1 antibody VK-8. The original mouse IgG1 antibody, VK-8, was used in the radioimmunoprecipitation of antigens from [3H]GlcN- labeled OVCAR-3 cells. Inhibition and indirect ELISA was used to test the effect of various enzymic and chemical treatments on binding of the IgG1 version of this antibody towards CA125. This antibody was also used in the identification of CA125 antigen in a western blot assay (PMID: 9180155). This antibody was used for the immunofluorescent staining of SW626/B53 cells (PMID: 11920644). This antibody has also been reported to be used in the affinity purification CA 125 antigen (PMID: 9180155; 11369781). This antibody was used detect sMUC16 in the peripheral blood mononuclear cells isolated from healthy pregnant women and preeclampsia patients at the time of delivery using flow cytometry (PMID: 22380506). This antibody can also be used for the immunohistochemical analysis on sections of corneal and conjunctival epithelia (PMID: 12766047).
Antibody First Published
Lloyd et al. Isolation and characterization of ovarian cancer antigen CA 125 using a new monoclonal antibody (VK-8): identification as a mucin-type molecule. Int J Cancer. 1997 May 29;71(5):842-50. PMID:9180155
Note on publication
Describes the use of this antibody in the characterization of ovarian cancer antigen CA 125.
Size
249 μg Purified antibody.
Concentration
150 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 449.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 169⁰C.
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