Cat. No.
MABL-3779
Application
FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
B4
From
Recombinant Antibody
Specificity
This antibody reacts with the B4 antigen present on the peripheral blood cells and lymphoid organs, on cell lines derived from normal and malignant B cells, and on tumor cells isolated from patients with B cell-derived neoplasms
Alternative Names
B4 antigen; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12
UniProt
P15391
Immunogen
The original antibody was generated against CD19 antigen present on human B cells.
Application Notes
This antibody is recommended to be used for the cell surface expression of the B4 antigen. It is reported that in mitogen stimulated B cells the expression of the B4 antigen increases initially with the B cell activation and then is lost with the B cell differentiation (PMID: 6408173). Conjugate of DC1 with murine and humanizsed versions of anti-B4 antibody was found to be potent and effective in selective killing of target cells in culture. It is also reported that the anti-B4-DC1 conjugate showed antitumor efficacy in an aggressive metastatic human B-cell lymphoma survival model in SCID mice and completely cured animals hearing large tumors (PMID: 7545085). Anti-B4 when conjugated with blocked ricin increased the antibody induced cell calcium mobilization and CD19 internalization. Modulation of CD19 induced by anti-B4 or anti-B4–bR was evaluated by indirect immunofluoresecence and flow cytometry (PMID: 9310487). Anti- B4-blocked ricin (anti-B4-bR) recognizes CD19-positive cells, which includes most B-cell non-Hodgkin’s lymphomas (NHLs). Phase II clinical studies of anti-B4-bR suggested that it does not actively penetrate in certain sites of the disease and is not an active agent in relapsed NHLs (PMID: 9829722)
Antibody First Published
Nadler et al. B4, a human B lymphocyte-associated antigen expressed on normal, mitogen-activated, and malignant B lymphocytes. J Immunol (1983);131(1):244-50. PMID:6408173
Note on publication
Describes the characterization of the B cell specific B4 antigen using this antibody.
Size
260 μg Purified antibody.
Concentration
161 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 460.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 180⁰C.
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