Cat. No.
MABL-3781
Application
ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
CH1A1A (2F1, CH1A1A-2F1)
From
Recombinant Antibody
Specificity
This antibody is capable of recognizing a membrane-proximal epitope in the B3 domain of CEA (CEACAM5, Cd66e), whereas it does not recognize shed CEA or soluble CEA as found in the circulation (PMID: 19904275; 7514303; 11517330).
Alternative Names
CD66e; CEACAM5; Carcinoembryonic antigen; Carcinoembryonic antigen- related cell adhesion molecule 5; Meconium antigen 100 ; PR1A3; PR1.A3
UniProt
P06731
Immunogen
The original murine antibody PR.1A3 was generated by immunizing BALB/c mice with mucosal scrapings and cell membrane preparations from normal large intestine. Booster dose was given with same antigen or in one case boosting was also performed with HT29 colon carcinoma cells. CH1A1A was later one generated for affinity maturation and stability enhancement by phage display.
Application Notes
CH1A1A is a humanized, affinity matured, and stability-engineered version derived from the murine parental PR1A3 antibody, first disclosed in PMID: 2434440. This antibody is also basis of CEA TCB (RG7802), a CEA-CD3 T cell bispecific antibody currently in Phase 1/1b clinical trials (PMID: 27622073; 27117182; 26861458). This antibody was also used in the generation of cergutuzumab amunaleukin (CEA-IL2v, RG7813), a monomeric CEA-targeted immunocytokine, that comprises a single IL-2 variant (IL2v) moiety with abolished CD25 binding, fused to the C-terminus of a high affinity, bivalent carcinoembryonic antigen (CEA)-specific antibody devoid of Fc mediated effector functions. The binding affinity of this antibody for CEA was tested using ELISA. This antibody can also be used in the immunohistochemical analysis of CEA expression by various tumors (PMID: 28405498). This antibody was also used to study the surface expression heterogeneity of CEA in cells extracted from patient derived colorectal cancer organoids (PDOs) using flow cytometry (PMID: 30982469).
Antibody First Published
Richman et al. Monoclonal antibodies to human colorectal epithelium:markers for differentiation and tumour characterization. Int J Cancer. 1987 Mar 15;39(3):317-28. PMID:2434440
Note on publication
Size
262 μg Purified antibody.
Concentration
163 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 462.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 182⁰C.
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