Anti-Cytokeratin 7 [OV-TL 12/30] (MABL-3788)

MABL-3788

WB, FC, IF, IHC

Engineer antibody

Human

OV-TL 12/30

Recombinant Antibody

This antibody recognizes cytokeratin-7. Cytokeratins are a subfamily of intermediate filament proteins but this antibody does not recognize other intermediate filament proteins. The antibody reacts with proteins that are found in most ductal, glandular, transitional, and biliary duct epithelial cells.

CK-7; Keratin, type II cytoskeletal 7; Cytokeratin-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7; CK7; CK 7

P08729

This antibody was originally raised by immunizing BALB/c mice with ovarian carcinoma cell line OTN11.

Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometry. Cytokeratin 7 (CK7) labeling can help distinguish between lung, breast carcinomas, and urothelial carcinomas that typically stain positive, and colon and prostate carcinomas that typically lack CK7 expression (Jerome et al., 2004; Logani et al., 2003; Murray et al., 2004; Roy et al., 2011). CK7 is a common marker of primary lung adenocarcinomas (almost all cases) with a lower specificity since it is also observed in other primary lung carcinomas and non-pulmonary carcinomas (Jerome et al., 2004). Anti-cytokeratin 7 has also been useful in the differential diagnosis of ovarian neoplasms (McCluggage et al., 2005). Furthermore, this antibody is a marker for distinguishing between the conjunctival and corneal surface epithelia which may be used in the diagnosis of limbal stem cell deficiency (LSCD) (Jirsova et al., 2011; pmid: 21693612). Finally, OV-TL 12/30 has proven very effective and popular in immunohistochemistry; for instance, one group used it to label cytokeratin-7 in human term placental samples (König et al., 2014; pmid: 24326460).

Ramaekers et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol. 1990 Mar; 136(3): 641–655. PMID:1690512

269 μg Purified antibody.

170 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 469.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 189⁰C.