Cat. No.
MABL-3789
Application
IP, radioimmunotherapy, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
TS1
From
Recombinant Antibody
Specificity
This antibody binds a discontinuous epitope on helical 2B domain of the human cytokeratin 8 between amino acid residues 340–365 (QRGELAIKDANAKLSELEAALQRAKQ). Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Together with KRT19, cytokeratin 8 helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Cytokeratin 8 is a serological tumor marker
Alternative Names
CK8; CK-8; CK 8; KRT8; CYK8; Keratin type II cytoskeletal 8; Cytokeratin- 8; Type-II keratin Kb8; TS 1; Mab 202
UniProt
P05787
Immunogen
The original antibody was generated by immunizing BALB/c mice with purified human gastrointestinal tumor derived cytokeratin.
Application Notes
This antibody has specific reactivity for human cytokeratin 8. The binding characterization and epitope mapping of this antibody was done using ELISA (PMID: 9486565; 9892182). This antibody was also capable of immunoprecipitating human cytokeratin 8 from a sample. This antibody can be used in the identification of cytokeratin 8 in a western blot assay. This antibody was also used in the immunohistochemical staining of frozen sections of normal human adult tissues like skin, esophagus and liver (PMID: 2479674). This antibody is capable of binding cytokeratin 8 expressed on carcinomas in vivo.This antibody was used for the radio immunolocalization of cytokeratin 8 and radioimmunotherapy (PMID: 9892182; 7493362; 9406704).
Antibody First Published
Sundström et al. Diversity in immunoreactivity of tumor-derived cytokeratin monoclonal antibodies. J Histochem Cytochem. 1989 Dec;37(12):1845-54. PMID:2479674
Note on publication
Describes the generation and characterization of 10 antibodies against cytokeratin which are expressed on normal and malignant epithelial cells.
Size
270 μg Purified antibody.
Concentration
171 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 470.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 190⁰C.
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