anti-deltaegfr-dh83-mabl-3790

Cat. No.

MABL-3790

Application

IP, WB, ELISA, FC, IHC

Isotype

Engineer antibody

Species Reactivity

Human

Clone No.

DH8.3

From

Recombinant Antibody

Specificity

This antibody is specific for ΔEGFR (EGFR type III), a truncated form of the epidermal growth factor receptor that is commonly associated with cancer cells, especially glioblastoma cells. ΔEGFR lacks exons 2–7 of the external domain. This antibody does not cross-react with full-length EGFR.

Alternative Names

ΔEGFR; Epidermal growth factor receptor; EC:2.7.10.1; Proto-oncogene c- ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR; ERBB; ERBB1; HER1

UniProt

P00533

Immunogen

This antibody was raised by immunising BALB/c mice with a synthetic peptide, known as 79E (LEEKKGNYVVTDHC), corresponding to the junctional sequence of the Δ2-7 EGFR deletion mutation.

Application Notes

This antibody has been used in fluorescence-activated cell sorting, Western blot and immunohistochemical analysis, using acetone-fixed frozen sections, of a tumour panel (Jungbluth et al, 2003). It does not stain normal tissues, which express wild-type EGFR (Jungbluth et al, 2003), and preferentially stains tumours expressing mutant receptors (Hills et al, 1995). This antibody has also been used in immunoprecipitation experiments (Hills et al, 1995) and ELISA analysis (Johns et al, 2002). This antibody has been shown to induce rapid internalisation of truncated EGFR by target cells (Johns et al, 2002), which could have therapeutic applications, given the tumour-restricted expression of ΔEGFR and its role in tumorigenesis.

Antibody First Published

Hills et al. Specific targeting of a mutant, activated FGF receptor found in glioblastoma using a monoclonal antibody. Int J Cancer. 1995 Nov 15;63(4):537-43. PMID:7591264

Note on publication

Describes the original generation of this antibody, and characterisation of its specificity in IP and WB experiments.

Size

271 μg Purified antibody.

Concentration

172 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 471.

Storage Recommendation

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 191⁰C.

Anti-deltaEGFR [DH8.3] (MABL-3790)

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