Cat. No.
MABL-3791
Application
FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
2224
From
Recombinant Antibody
Specificity
This antibody is specific for human EGFR.
Alternative Names
ERBB1; ERBB; Erb-B2; HER1; Epidermal growth factor receptor; Proto- oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
UniProt
P00533
Immunogen
The original antibody was created using phage display from large naïve human scFv libraries.
Application Notes
This antibody is derived after affinity maturation of a parental anti-EGFR clone C10. The original scFv antibody showed a 280 fold increase in affinity compared to the parental scFv clone C10. This antibody was used for analysis of scFv-mediated internalization of fluorescent immunoliposomes and quantification by microscopy and flow cytometry. This antibody bound to human EGFR expressed on MDAMB468 cells, A431 cells and EGFR (vIII) stably transfected NR6M and was detected by flow cytometry (Zhou et al, 2007; pmid:17602702).
Antibody First Published
Zhou et al. Impact of single-chain Fv antibody fragment affinity on nanoparticle targeting of epidermal growth factor receptor-expressing tumor cells. J Mol Biol. 2007 Aug 24;371(4):934-47. PMID:17602702
Note on publication
A library containing scFv mutants was created by error-prone PCR, displayed on the surface of yeast, and higher affinity clones selected by fluorescence activated cell sorting.
Size
272 μg Purified antibody.
Concentration
173 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 472.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 192⁰C.
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