Cat. No.
MABL-3798
Application
WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
S4 10H
From
Recombinant Antibody
Specificity
Alternative Names
Epidermal growth factor receptor; ERBB; ERBB1; mENA; PIG61; NISBD2
UniProt
P00533
Immunogen
Human EGFR.
Application Notes
The antibody binds specifically to EGFR, a cell-surface receptor which is part of the ErbB family of receptors. The EGFR is a receptor tyrosine kinase, which, upon activation by its ligands, such as epidermal growth factor and transforming growth factor alpha, triggers signal transduction pathways that increase cell migration, adhesion and proliferation. Mutations that lead to the overexpression of EGFR are associated with the development of cancer. The antibody was prepared from phage antibodies isolated from the spleen library.
Antibody First Published
Kettleborough et al. Isolation of tumor cell-specific single-chain Fv from immunized mice using phage-antibody libraries and the re-construction of whole antibodies from these antibody fragments. Eur. J. Immunol. 24 (4), 952-958 (1994) PMID:8149964
Note on publication
Describes the phage-antibody technology, which is used to produce three phage- antibody libraries from which mouse anti-EGFR scFv antibodies are generated. The libraries are prepared from the spleen, lymph node and in vitro cells of an immunized mouse. The antibodies produced are compared with a mouse mAb, 425, isolated by standard hybridoma technology.
Size
279 μg Purified antibody.
Concentration
180 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 479.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 199⁰C.
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