Cat. No.
MABL-3799
Application
Biacore, WB, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
L8A4
From
Recombinant Antibody
Specificity
Binds EGFRvIII, a mutated form of EGFR, plays a prominent role in tumorigenesis.
Alternative Names
Epidermal Growth Factor Receptor variant III
UniProt
P00533
Immunogen
Pep 3-KLH (Pep 3 is a 14-amino acid peptide corresponding to the predicted amino acid sequence at the fusion junction and KLH stands for keyhole limpet hemocyanin) at da 40 and 103, HC2 20 d2 cells at day 1, 25, 74, 87. And fusion with the nonimmunoglobulin-secreting Kearney variant of P3X63/Ag8.653.
Application Notes
This antibody can be used to detect EGFRvIII, a mutated EGFR variant, in immunohistochemical staining, Western Blot, ELISA and flow-cytometry. Chou et al (2016, PMID: 27825958) used this antibody to detect EGFRvIII in acetone and PFA fixed sections by fluorescent immunohistochemistry.
Antibody First Published
Wikstrand CJ, Hale LP, Batra SK, Hill ML, Humphrey PA, Kurpad SN, McLendon RE, Moscatello D, Pegram CN, Reist CJ, et al. Monoclonal antibodies against EGFRvIII are tumor specific and react with breast and lung carcinomas and malignant gliomas. Cancer Res. 1995 Jul 15;55(14):3140-8. PMID:7606735
Note on publication
Describes the preparation anti human EGFRvIII antibody by long-termimmunization protocols using the EGFRvIII-specific synthetic peptide and the intact variant. Identified using ELISA, Western blot, flow cytometry, and immunohistochemistry techniques.
Size
280 μg Purified antibody.
Concentration
181 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 480.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 200⁰C.
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