Cat. No.
MABL-3812
Application
SPR, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
A5
From
Recombinant Antibody
Specificity
This antibody is specific for HER3.
Alternative Names
ERBB3; Receptor tyrosine-protein kinase erbB-3; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3; HER3.A5
UniProt
P21860
Immunogen
The original antibody was created using phage display from large naïve human scFv libraries.
Application Notes
A5 scFv binds to ErbB3 ECD with a KD of 1.6 × 10−7 M. The ability to bind HER3 was assesed in this antibody using surface plasmon resonance. An anti ErbB2/ErbB3 bispecific antibody made from ML3-9 and A5 clones called the A5-linker-ML3.9 bs-scFv exhibits selective targeting of tumour cells in vitro and in vivo that co-express the two target antigens over tumour cells that express only one target antigen or normal cells that express low levels of both antigens. This study was done using flow cytometry. (Robinson et al, 2008;pmid:18841159).
Antibody First Published
Horak et al. Isolation of scFvs to in vitro produced extracellular domains of EGFR family members. Cancer Biother Radiopharm. (2005); 20(6):603-13. PMID:16398612
Note on publication
Describes the generation and characterization of scFvs against extracellular domain of the EGFR family proteins.
Size
293 μg Purified antibody.
Concentration
194 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 493.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 213⁰C.
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