Cat. No.
MABL-3824
Application
IP, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
11-25
From
Recombinant Antibody
Specificity
This antibody binds the human mesothelin protein, which is highly expressed in many human cancers, including the majority of pancreatic adenocarcinomas, ovarian cancers, and mesotheliomas.
Alternative Names
MSLN; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor
UniProt
Q13421
Immunogen
The original antibody was generated by immunizing BALB/c mice with the purified recombinant mesothelin protein.
Application Notes
It is recommended that this antibody be used in combination with mAb IC14-30 to detect mesothelin with high sensitivity in a capture ELISA. This antibody can also immunoprecipitate recombinant mesothelin. NCI-H226 and NCI-H520 were incubated with anti-MSLN antibodies (11—25) or an isotype-matched control antibody and followed by PE-conjugate anti-mouse IgG. Antigen expression was detected by flow cytometry (Iwahori et al., 2008; PMID: 18394747). 64Cu labeled 11-25 was used in vitro cell binding assay and in vivo positron emission tomography (PET) imaging in the tumor-bearing mice (Kobayashi et al., 2015; PMID: 25883990).
Antibody First Published
Iwahori et al., Megakaryocyte potentiating factor as a tumor marker of malignant pleural mesothelioma: evaluation in comparison with mesothelin. Lung Cancer. 2008 Oct;62(1):45-54. doi: 10.1016/j.lungcan.2008.02.012. PMID:18394747
Note on publication
The original publication evaluates the diagnostic efficacy of megakaryocyte potentiating factor (MPF) compared to mesothelin (MSLN) as serum biomarkers for malignant pleural mesothelioma (MPM), using a novel ELISA system with developed antibodies, including clone 11-25.
Size
305 μg Purified antibody.
Concentration
206 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 505.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 225⁰C.
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