Cat. No.
MABL-3823
Application
IP, WB, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
21B4
From
Recombinant Antibody
Specificity
This antibody binds human MAGEA3 and MAGEA6, but does not cross react with other members of the MAGE family like MAGE-A1, MAGE-A4 and MAGE-A10. MAGE-A3 is a tumor specific antigen and it is expressed on many tumors like melanoma, non-small cell lung cancer, hematologic malignancies, and others.
Alternative Names
MAGE-3; MAGE3; MAGE 3; CT1.3; MAGE-3 antigen; Melanoma-associated antigen 3; Antigen MZ2-D; Cancer/testis antigen 1.3; MAGEA6; MAGE6; Melanoma-associated antigen 6; Cancer/testis antigen 1.6; CT1.6; MAGE-6 antigen; MAGE3B antigen
UniProt
P43357; P43360
Immunogen
The original antibody was isolated from a patient suffering from a tumor expressing a tumor specific antigen like MAGE-A3.
Application Notes
The binding specificity of this antibody towards MAGE-A3 was determined using ELISA. The original antibody cross reacts with MAGEA6 but does not react with other members of the MAGE family in an ELISA or western blot assay. This antibody was used in the detection of HEK293T cells expressing MAGEA3 and MAGEA6 using flow cytometry. The original IgG1 antibody was capable of immunoprecipitating MAGE-A3 from lysates of SK-MEL-37 cells endogenously expressing MAGEA3. The original antibody was also used in the immunohistochemical staining of tissues form normal testis and squamous cell carcinoma of the lung (US20140093514).
Antibody First Published
Esslinger et al. Magea3 binding antibodies. (US20140093514)
Note on publication
Size
304 μg Purified antibody.
Concentration
205 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 504.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 224⁰C.
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