Cat. No.
MABL-3822
Application
ICC, IPP, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
FMC-9
From
Recombinant Antibody
Specificity
The antibody binds to m5dCyd with an IC50 of 4.27 μg/ml.
Alternative Names
5-methylcytosine
UniProt
Immunogen
m5Cyd-KLH conjugate.
Application Notes
The antibody binds specifically to 5-Methylcytosine, a methylated form of the nucleobase cytosine, a component of both DNA and RNA. In DNA, the presence of 5mC is an epigenetic mark for genome stability during embryonic development. In RNA, 5-methylcytidine is associated with structural stability, aminoacylation and codon recognition of tRNA. The monoclonal antibody FMC9 can be used for detection of methylated DNA in patient blood serum, and can be detected in the urine or tissue of patients with certain cancers, including bladder, breast and colon.
Antibody First Published
Mizugaki M, Itoh K, Yamaguchi T, Ishiwata S, Hishinuma T, Nozaki S, Ishida N. Preparation of a monoclonal antibody specific for 5-methyl-2'-deoxycytidine and its application for the detection of DNA methylation levels in human peripheral blood cells. Biol Pharm Bull. 1996 Dec;19(12):1537-40. PMID:8996634
Note on publication
Describes the preparation of the monoclonal antibody FMC9, specific for the modified nucleoside 5-methyl-2'-deoxycytidine (m5dCyd). The antibody was prepared using 5- methylcytidine -keyhole limpet haemocyanin (KLH) conjugate. Also describes how the FMC9 antibody can be used in monitoring m5dCyd levels in peripheral blood cells.
Size
303 μg Purified antibody.
Concentration
204 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 503.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 223⁰C.
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