Cat. No.
MABL-3821
Application
inhibit, IP, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Epstein-Barr virus (EBV)
Clone No.
H3
From
Recombinant Antibody
Specificity
H3 scFv is specific to the LMP1 C terminal region where it binds to CTAR-1 (GST-LMP1[187- 242]). LMP1 is expessed constitutively in many EBV-associated tumours and this protein can cause B lymphocyte and rodent fibroblast transformation, cell apoptosis inhibition by up-regulation of anti-apoptotic proteins, cell surface marker and DNA methyltransferase activty up-regulation, and cell adhesion molecule and cyclin-dependent kinase downregulation. The C-terminal domain of LMP1 is made up of 3 C-terminal activating regions (CTARs) which interact with TNF receptor-associated proteins and TNF receptor- associated death domain protein, which medite activation of NF-kB and AP-1. A LMP1 TAR is also involved in the JAK/STAT pathway.
Alternative Names
latent membrane protein 1; LMP1; LMP-1; p63; protein p63; protein p25
UniProt
P03230
Immunogen
H3 was selected by its affinity for GST-LMP1 from a human phage display library after 4 rounds of panning.
Application Notes
H3 scFv can reduce LMP1-mediated NF-kappaB activation in HEK293 cells by ~50% as well as inhibit LMP1 functions in epithelial cells - enables the potential use of H3 for attenuating the LMP1 function in LMP1-positive tumors. H3 is also capable of reducing the trans-membrane migration ability of MDCK-LMP1 cells by blocking the CTAR-mediated signaling pathway. H3 can also be used to pull down LMP1 in IPs, and can be used for ELISAs and to visualise LMP1 distibution in IF studies.
Antibody First Published
Fang et al. Modulation of Epstein-Barr virus latent membrane protein 1 activity by intrabodies. Intervirology. 2007;50(4):254-63. Epub 2007 Apr 25. PMID:17460414
Note on publication
Describes the generation of and characterization of a number of scFvs specific for different regions of the LMP1 C terminal domain.
Size
302 μg Purified antibody.
Concentration
203 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 502.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 222⁰C.
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