Cat. No.
MABL-3833
Application
IP, IRA, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Mc5
From
Recombinant Antibody
Specificity
This antibody is specific for the amino acid sequence DTRPAP of MUC1. The alpha subunit of MUC1 has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit of MUC-1 contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein- protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53- mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
Alternative Names
CD227; BEM; Breast epithelial mucin; Mucin-1; Breast carcinoma- associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin
UniProt
P15941
Immunogen
Human breast epithelial mucin.
Application Notes
In an attempt to develop a reliable way to detect breast cancer, immunoprecipitation was preformed on NPGP from HMFG using the mouse version of this antibody bound to Sepharose 4B. Furthermore, this antibody was tested in an radioimunnology experiment on NPGP using the mouse version of this antibody (Ceriani et al, 1992; pmid:1377884). The epitope of this antibody was mapped by ELISA using small peptides - the murine version of this antibody was used (Petrakou et al, 1998 ; pmid:9422085). This antibody was used to identify cells expressing the MUC1 protein. This was accomplished by using the mouse version of this antibody on cell lysates of cell transfected with the MUC1 protein in a western blot analysis (Rubinstein et al, 2006; pmid:17145869). To compare the diagnostic usefulness of different antibodies, the mouse version of this antibody was used for immunohistochemistry on cell pellets from pleural fluid specimens (Creaney et al, 2008; pmid:18454162). While developing an antibody against the alpha/beta junction of MUC1 the mouse version of this antibody was used for western blot on the MUC1 protein (Rubinstein et al, 2008; pmid:18821582).
Antibody First Published
Christian et al. 1996 Cloning and expression of cDNAs encoding the variable domains of the anti-breast carcinoma antibody Mc5. Hybridoma 1996; 15(2):155-158 PMID:8743296
Note on publication
Describes how Mc5 has been used as a reagent in the diagnosis of breast cancer.
Size
314 μg Purified antibody.
Concentration
215 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 514.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 234⁰C.
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