Cat. No.
MABL-3834
Application
therapeutic, WB, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
PAM4
From
Recombinant Antibody
Specificity
This antibody is specific for the PAM4 domain of the human MUC1 protein.
Alternative Names
Mucin-1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut- reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; CD227
UniProt
P15941
Immunogen
The original antibody was raised by immunization of mice with the PAM4 antigen.
Application Notes
This antibody was used for immunohistochemistry on normal adult tissues and neoplastic tissues. This yielded no to weak staining in normal tissue and stronger staining in 85% of the neoplastic tissue (US7238786). ELISA was performed on blood serum of healthy humans, pancreatic cancer patients, pancreatitis patients, ovarian cancer patients and breast cancer patients (US7238786). This antibody was used to treat mice that were given the CaPanl tumor. The animals showed regression of tumor (US7238786). ELISA was performed on blood sera of cancer patients using this antibody (Gold et al, 2005; pmid:16344318). Immunohistochemistry was performed on 63 PanIN and 36 IPMN lesions using this antibody (Gold et al, 2007; pmid:18094420). Flow cytometry was performed on the cell lines: RPMI 8226, U266, MC/CAR JJN-3, KMS12-BM, KMS 12-PE, DUL4, MUC1, breast carcinoma cell lines, MCF7, MDA-MB468, T47D, ZR-75-30, and a pancreatic carcinoma cell line, CaPan-1. This was done using this antibody (Burton et al, 1999; pmid:10541345). A western blot was performed using this antibody (Burton et al, 1999; pmid:10541345).
Antibody First Published
Gold et al. PAM4-reactive MUC1 is a biomarker for early pancreatic adenocarcinoma. Clin Cancer Res. 2007 Dec 15;13(24):7380-7. PMID:18094420
Note on publication
The expression of the PAM4-reactive MUC1 in the noninvasive precursor lesions, pancreatic intraepithelial neoplasia (PanIN) and intraductal papillary mucinous neoplasia (IPMN) was examined.
Size
315 μg Purified antibody.
Concentration
216 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 515.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 235⁰C.
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