anti-osteosarcoma-associated-cell-membrane-antigen-tp-1-mabl-3839

Cat. No.

MABL-3839

Application

radioimmunodetection, IF, IHC

Isotype

Engineer antibody

Species Reactivity

Dog, Human

Clone No.

TP-1

From

Recombinant Antibody

Specificity

This osteosarcoma-specific antibody binds an epitope on a alkaline phosphatase isoform expressed on both canine and human osteosarcoma cells, but with very limited expression on normal cells and other cancer forms. It also reacts with some other major subtypes of human sarcomas.

Alternative Names

UniProt

Immunogen

This antibody was raised by immunising BALB/c mice with tumor cells from a human osteosarcoma, TPX, grown as a xenograft in athymic BALB/c nude mice.

Application Notes

In indirect immunofluorescence assays, this antibody has been show to bind to the surface of human osteosarcoma TPX cells, but not to autologous skin fibroblasts, non-sarcomatous tumor cells or peripheral blood lymphocytes (Bruland et al, 1986).In IHC analysis, this antibody has been shown to bind to osteosarcomas, along with other major human sarcoma subtypes, including synovial sarcomas, malignant fibrous histiocytomas, malignant hemangiopericytomas and chondrosarcomas. It does no react with liposarcomas and leio- and rhabdomyosarcomas, or non-sarcomatous malignancies or normal tissue (Bruland et al, 1986). This binding profile matches that of MAb TP-3. A radiolabelled Fab derived from this antibody localises to human osteogenic sarcoma xenografts after intravenous injection (Bruland et al, 1987). In a pilot study, this antibody showed potential as a immunoscintigraphic agent for the early detection of lung metastases in patients with bone sarcoma (Bruland et al, 1994).

Antibody First Published

Bruland et al. New monoclonal antibodies specific for human sarcomas. Int J Cancer. 1986 Jul 15;38(1):27-31. PMID:3522438

Note on publication

Describes the original generation of this antibody, and its characterisation.

Size

320 μg Purified antibody.

Concentration

221 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 520.

Storage Recommendation

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 240⁰C.

Anti-osteosarcoma-associated cell membrane antigen [TP-1] (MABL-3839)

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