Cat. No.
MABL-3840
Application
radioimmunodetection, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Dog, Human
Clone No.
TP-3
From
Recombinant Antibody
Specificity
This osteosarcoma-specific antibody binds an epitope on an alkaline phosphatase isoform expressed on both canine and human osteosarcoma cells, but with very limited expression on normal cells and other cancer forms. It also reacts with some other major subtypes of human sarcomas.
Alternative Names
UniProt
Immunogen
This antibody was raised by immunising BALB/c mice with tumor cells from a human osteosarcoma, TPX, grown as a xenograft in athymic BALB/c nude mice.
Application Notes
In indirect immunofluorescence assays, this antibody has been show to bind to the surface of human osteosarcoma TPX cells, but not to autologous skin fibroblasts, non-sarcomatous tumor cells or peripheral blood lymphocytes (Bruland et al, 1986).In IHC analysis, this antibody has been shown to bind to osteosarcomas, along with other major human sarcoma subtypes, including synovial sarcomas, malignant fibrous histiocytomas, malignant hemangiopericytomas and chondrosarcomas. It does not react with liposarcomas and leio- and rhabdomyosarcomas, or non-sarcomatous malignancies or normal tissue (Bruland et al, 1986). This binding profile matches that of MAb TP-1. A radiolabelled version of this antibody localises to human osteogenic sarcoma xenografts after intravenous injection (Bruland et al, 1987).
Antibody First Published
Bruland et al. New monoclonal antibodies specific for human sarcomas. Int J Cancer. 1986 Jul 15;38(1):27-31. PMID:3522438
Note on publication
Describes the original generation of this antibody, and its characterisation in IF and IHC analysis.
Size
321 μg Purified antibody.
Concentration
222 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 521.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 241⁰C.
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