Cat. No.
MABL-3841
Application
IP
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
Y13-238
From
Recombinant Antibody
Specificity
The antibody reacts with v-H-ras (within amino acids 120-138) and rodent c-H-ras p21′s but not v-K-ras or rodent c-K-ras p21′s. This antibody also recognizes human c-H-ras and c-K-ras p21′s, but not human c-N-ras.
Alternative Names
Ras; p21ras;
UniProt
P78460; O70564
Immunogen
Injection of rats subcutaneously with Ha-MuSV transformed NRK cells and screening of sera of individual animals to identify those with the strongest immune response against p21. Spleens were removed from the immunised animals and cell fusion of immune lymphocytes with rat myeloma cell line 210RCY3 Ag1.2.3 (Y3) was carried out. Supernatants were harvested and assayed for antibodies to p21 by GDP binding assay or immunofluorescence on Ha-SV-transformed MDCK cells, followed by testing for the ability of the antibodies to immunoprecipitate p21.
Application Notes
Y13-238 has been used to immunoprecipitate p21 from human peripheral blood lymphoblasts in order to measure its activation state (Downward 1990). Y13-238 has been used as an anti- ras non-neutralising scFv (Cochet 1998).
Antibody First Published
Furth et al. Monoclonal Antibodies to the p21 Products of the Transforming Gene of Harvey Murine Sarcoma Virus and of the Cellular ras Gene Family JOURNAL OF VIROLOGY, July 1982, p. 294-304 PMID:6287003
Note on publication
Describes the generation of monoclonal antibodies to p21 encoded by Ha-MuSV.
Size
322 μg Purified antibody.
Concentration
223 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 522.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 242⁰C.
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