Cat. No.
MABL-3842
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
DWP
From
Recombinant Antibody
Specificity
This antibody recognizes human mutant H-, K-, and N-Ras proteins. This antibody may also react with other Ras mutants with a hydrophobic amino acid (V, A, C, H, I, L, M, Y, T, N, Q) at position 12 (PMID: 9440050). This ras protein binds GDP/GTP and possess intrinsic GTPase activity, that results in conversion of nucleotide guanosine triphosphate (GTP) into guanosine diphosphate (GDP). This protein plays an important role in many signal transduction pathways and regulation of cell proliferation. Somatic ras mutations are highly observed in leukemias, colorectal cancer, pancreatic cancer and lung cancer.
Alternative Names
p21; p21 ras protein; Ras; pan ras; KRAS G12V; K-Ras; GTPase Kras; KRAS2; RASK2; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; ras p21 protein; p21 ras; GTPase Hras; HRAS; H-RAS; HRAS; HRAS1; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; N-RAS; NRAS; HRAS1; GTPase NRas; Transforming protein N-Ras; DWP-1-1-3
UniProt
P01116; P01112; P01111
Immunogen
The original antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 5-16 (KLVVVGAVGVGK) of a mutated RAS gene encoding Val instead of Gly at position 12 coupled to the carrier protein bovine thyroglobulin (BTG) and to keyhole limpet hemocyanin (KLH).
Application Notes
This antibody was capable of binding G12V and G12C mutant peptides of human ras protein in competition ELISAs (PMID: 3094010). It was reported to cross react with peptides containing amino acids V, A, C, H, I, L, M, Y, T, N, Q at position 12 in an ELISA (PMID: 9440050). This antibody could detect mutant KRAS proteins from cell lysates of human tumor cell lines in a western blot, but did not react with normal KRAS or other KRAS mutants. Immunohistochemical studies showed that DWP-labeled transformed NIH cells and human carcinoma cells contained p21ras is with either Val or Cys at position 12 but not normal or other activated KRAS protein (PMID: 3094010).
Antibody First Published
Carney et al. Monoclonal antibody specific for an activated RAS protein. Proc Natl Acad Sci U S A. 1986 Oct;83(19):7485-9. PMID:3094010
Note on publication
This paper describes the generation and characterization of an antibody that recognizes mutated RAS protein.
Size
323 μg Purified antibody.
Concentration
224 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 523.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 243⁰C.
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