Cat. No.
MABL-3843
Application
ICC, IHC-FFPE, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
KGH-R1
From
Recombinant Antibody
Specificity
This antibody recognizes human wild-type and mutant H-, K-, and N-Ras proteins. This ras protein binds GDP/GTP and possess intrinsic GTPase activity, that results in conversion of nucleotide guanosine triphosphate (GTP) into guanosine diphosphate (GDP). This protein plays an important role in many signal transduction pathways and regulation of cell proliferation. Somatic ras mutations are highly observed in leukemias, colorectal cancer, pancreatic cancer and lung cancer.
Alternative Names
p21; p21 ras protein; Ras; pan ras; K-Ras; GTPase Kras; KRAS2; RASK2; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; ras p21 protein; p21 ras; GTPase Hras; HRAS; H-RAS; HRAS; HRAS1; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; N-RAS; NRAS; HRAS1; GTPase NRas; Transforming protein N-Ras
UniProt
P01116; P01112; P01111
Immunogen
The original antibody was generated by immunizing BALB/c mice with H-p21Ras was used as an immunogen.
Application Notes
The binding reactivity of this antibody to H-p21Ras, K-p21Ras and N-p21Ras protein was determined using ELISA. This antibody was also capable of recognizing wild type and mutated ras protein from various normal and tumor cell lines using western blot and immunocytometry assay. This antibody presented strong immunoreactivity for 14 primary human cancer tissues but showed weak or negative immunoreactivity in the corresponding normal tissues. It was reported to bind positively with QGY7703, SMMC7721, HepG2: hepatocarcinoma; BGC853, MKN28: gastric cancer; HCT116: colorectal cancer; T24: bladder cancer; SKOV3: ovary cancer; MDA-MB-231, MDA-MB-435, MCF7: breast cancer; HeLa: cervical cancer; Hep2: laryngocarcinoma; C8166, K562 cell lines (PMID: 26897358). This antibody was also used in the immunohistochemical staining of FFPE section of colorectal tissues, colorectal inflammatory polyps, colorectal low-grade intraepithelial neoplasia, colorectal high-grade intraepithelial neoplasia, invasive colorectal carcinomas and corresponding adjacent tissues. The immunoreactivity was measured by evaluating percentage of positive cells and histological score (HSCORE) (PMID: 21138685). This antibody can also be used in the immunofluorescent staining of various normal and cancer tissues (PMID: 32664770).
Antibody First Published
Yang et al. A novel anti-p21Ras scFv antibody reacting specifically with human tumor cell lines and primary tumor tissues. BMC Cancer. 2016; 16: 131. PMID:26897358
Note on publication
Describes the generation of an scFv antibody against p21ras protein and evaluates it binding reactivity with different human cancerous tissues.
Size
324 μg Purified antibody.
Concentration
225 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 524.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 244⁰C.
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