Cat. No.
MABL-3851
Application
WB, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
S1-61
From
Recombinant Antibody
Specificity
This antibody is specific against an epitope located on the alpha-chain of S100. S100 is a protein antigen present at certain cell types and useful in anatomy and pathology. It is capable of binding calcium and is similar to calmodulin but its exact function is unclear.
Alternative Names
S-100 protein; Protein S100-A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 calcium-binding protein A1; S-100
UniProt
P23297
Immunogen
This antibody was raised by immunising BALB/c mice with S-100 protein, which was isolated from bovine brain andconjugated to BSA.
Application Notes
This antibody is useful in determining the presence of S100 protein marker which might be used to characterise various normal and malignant cells. S1-61 antibody was initially used to immunohistochemically label cells in different tissues, such as nerve, striated muscle and melanoma, among others (Vanstapel et al., 1986). Another group utilised it to immunohistochemically identify strongly S100-positive extensions of central nervous system tissue in the area of dorsal root entry zones in patient samples with Friedreich ataxia (Koeppen et al., 2017). This antibody was shown to be also effective in detecting S100 on Western blot - one group analysed S100 expression in liver cells subjected to the activity of exosomes containing tumour-derived miRNA (Zeng et al., 2018).
Antibody First Published
Vanstapel et al. Production of monoclonal antibodies directed against antigenic determinants common to the alpha- and beta-chain of bovine brain S-100 protein. Lab Invest. 1985 Feb;52(2):232-8. PMID:2578587
Note on publication
This article describes generation and characterisation of S1-61 and (among other anti-S100 monoclonal antibodies).
Size
332 μg Purified antibody.
Concentration
233 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 532.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 252⁰C.
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