Anti-Botulinum neurotoxin mAbs

Cat. No.

MABL-030

Name

Anti-Botulinum neurotoxin mAbs

Clone No.

AFD-MCS6-27

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA

Species Reactivity

Clostridium botulinum

Basic Information

Specificity

/

Alternative Name

 BBoNT/B

UniProt

Immunogen

 Recombinant BoNT/B fragments expressed in E. coli as GST-fusion   proteins.

Application Notes

The antibody binds specifically to   BoNT/B, a two-chain neurotoxic protein produced by the bacterium Clostridium   botulinum. Botulism, an infection of the bacterium, which is commonly spread   through contaminated food, can be a fatal disease, and symptoms include a   gradual flaccid paralysis.Paralysis is caused by the internalization by   endocytosis of the toxin in specific axons, mediated by the attachment of the   heavy chain of the protein to axon surface. Once inside the axon, BoNT/B acts   by cleaving SNARE proteins, such as VAMP, which are involved in the docking   and fusion of neurosecretory vesicles. This prevents the release of the   neurotransmitter acetylcholine, resulting in paralysis of the muscles. The   monoclonal antibody MCS6-27 binds to the Hc and H5 fragments of BoNT/B, and   the binding epitope is located in the heavy chain, between amino acids E859   and E1291. The monoclonal antibody is able to capture BoNT/B at SDS   concentrations between 0 and 0.4 mM, and can be used to screen food samples   for botulinum toxin.

Antibody First Published

 Scotcher MC, Cheng LW, Stanker LH. Detection   of botulinum neurotoxin serotype B at sub mouse LD(50) levels by a sandwich   immunoassay and its application to toxin detection in milk. PLoS One. 2010   Jun 10;5(6). PMID:20548779

Note on publication

Describes the characterization of   monoclonal antibodies against BoNT/B, and the generation of a sensitive   sandwich immunoassay.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.