Anti-PrPSc mAbs

Cat. No.

MABL-050

Name

Anti-PrPSc mAbs

Clone No.

AFD-6H10

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, neutralising, ELISA

Species Reactivity

Cow, Sheep, Mouse

Basic Information

Specificity

This antibody is specific for PrPSc,   and binds to a conformational epitope that incudes the extreme C-terminus of   PrP. It does not react with recombinant mouse PrP or denatured PrPSc.

Alternative Name

Prion; prion protein; PrP; CD230; PRNP; PRP;   SIP

UniProt

P23907

Immunogen

The binding specificity of this antibody for   PrPSc has been confirmed in ELISA analysis; this antibody reacts with   non-denatured PrPSc, but not with recombinant mouse PrP or denatured PrPSc   treated with more than 3 M GdnHCl (Horiuchi et al, 2009). This antibody has   been shown to immunoprecipitate PrPSc from the brains of mice, sheep, and   cattle infected with prions (Horiuchi et al, 2009). Pretreatment of purified   PrPSc with this antibody results in a 1 log reduction in infectious titre   (Horiuchi et al, 2009).This antibody has also been used to identify   C-terminal conformational differences between prion strains (Saijo et al,   2016).As this antibody recognises a conformational epitope on PrPSc, it does   not react with PrPSc in immunoblot analysis (Horiuchi et al, 2009).

Application Notes

The binding specificity of this   antibody for PrPSc has been confirmed in ELISA analysis; this antibody reacts   with non-denatured PrPSc, but not with recombinant mouse PrP or denatured   PrPSc treated with more than 3 M GdnHCl (Horiuchi et al, 2009). This antibody   has been shown to immunoprecipitate PrPSc from the brains of mice, sheep, and   cattle infected with prions (Horiuchi et al, 2009). Pretreatment of purified   PrPSc with this antibody results in a 1 log reduction in infectious titre   (Horiuchi et al, 2009).This antibody has also been used to identify   C-terminal conformational differences between prion strains (Saijo et al,   2016).As this antibody recognises a conformational epitope on PrPSc, it does   not react with PrPSc in immunoblot analysis (Horiuchi et al, 2009).

Antibody First Published

 Horiuchi et al. Generation of monoclonal   antibody that distinguishes PrPSc from PrPC and neutralizes prion infectivity   Virology; Volume 394, Issue 2, 25 November 2009, Pages 200-207 PMID:19766283

Note on publication

Describes the original generation of   this antibody, and its characterisation.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.