Key features and details | |
Cat. No. | MABL-052 |
Name | Anti-MOG mAbs |
Clone No. | AFD-M38 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, ELISA, IHC |
Species Reactivity | Rat, Human |
Basic Information | |
Specificity | von Budingen et al Molecular characterization of antibody specificities against myelin/oligodendrocyte glycoprotein in autoimmune demyelination Proc. Natl. Acad. Sci. U.S.A. 99 (12), 8207-8212 (2002) |
Alternative Name | Myelin oligodendrocyte glycoprotein; rMOG |
UniProt | Q63346 |
Immunogen | Callithrix jacchus marmosets were used, and experimental allergic encephalomyelitis was induced by the injection of rat MOG (aa1-125) into the marmosettes. The rMOG was expressed in E. coli and purified to homogeneity. The animals were killed 4-70 days after the onset of symptoms of EAE. Bone marrow and spleen cells were obtained from an immunized C. jacchus, the RNA extracted with Trizol reagent and rtPCR used to generate cloning inserts containing Fab portions of IgGk. Phage display was then used to select for the MOG-reactive Fab fragments using the pCOMB3H phage display vector, and binding confirmed using an ELISA. |
Application Notes | Recognises a similar epitope as M26 and M45 as they have been shown to compete for binding. Also competes with M3-8 and so is likely to have overlapping epitopes. Recognises an epitope distinct from M3-31 or M2-24. The epitope recognised is a structural epitope, shown by the lack of recognition of linear MOG peptides. |
Antibody First Published | von Budingen et al Molecular characterization of antibody specificities against myelin/oligodendrocyte glycoprotein in autoimmune demyelination Proc. Natl. Acad. Sci. U.S.A. 99 (12), 8207-8212 (2002) PMID:12060766 |
Note on publication | An analysis of 6 Fab fragments against epitopes on MOG, identifying at least 4 separate epitopes. Analysis of these epitopes including competition assays with marmoset auto-antibodies,human auto-antibodies from MS patients, and confirmation that the epit |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
